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Microtomy - Microtomy is the cutting of paraffin embedded tissue block. The thickness of a section is normally 5 microns.

Processing - The procedure of removing the water from tissue and replacing it with paraffin.
Embedding - Placing the tissue into a block of paraffin.  This supports the tissue to allow it to be cut.

Grossing - Taking tissue from its fixative and placing it in a labeled cassette for processing

SPECIAL STAINS
- Class One Stains
Kinyoun’s AFB – acid fast bacilli
Alcian Blue – demonstrates acid mucopolysaccharides
Congo Red – amyloid
Giemsa – h.pylori
PAS /AB – differentiate between neutral & acidic mucosubstances
PAS w/digestion – demonstrates glycogen in tissue
PAS w/o digestion – demonstrates polysaccharides, neutral mucosubstances & basement membranes



Masson’s Trichrome – differentiate between collagen & smooth muscle
Verhoeff’s - elastic
Colloidal Iron – colloidal iron
Oil Red O – fat – frozen tissue only
Toluidine Blue – mast cells
Prussian Blue – ferric ironGram – gram + and gram -
Luxol Fast Blue - myelin sheath
Mayer’s Mucicarmine – epithelial mucin
Saffron O – detection of cartilage

IHC is available, please call the lab and speak with Jackie. Email: jsmith@amllabs.com or call us at 410-866-1200
All other special stains performed upon request

- Class Two Stains
Sevier-Munger – argyrophil cells
Grocott’s Methenamine Silver (GMS) – fungus
Von Kossa’s – calcium


DNA Slide Preparation
There is an additional charge for handling DNA projects, a new blade is used for each block to eliminate any cross contamination.  The charge is $3.00 per blade


Immunohistochemistry
At AML  Labs we use the ABC (avidin-biotin complex) method of IHC staining.  We found that this method had low background and is reported to be up to 40X more sensitive than that of other methods.  


Frozen Sections
We accept the frozen tissues samples mounted OCT and properly labeled with the specimen identification. Please call AML for mounting instructions. The tissue samples are then sent to AML Labs on dry ice priority overnight.

We purchase the molds and OCT from American Master Tech Scientific:    www.americanmastertech.com
This link will take you to Disposable Molds at American Master Tech and this link is the OCT



AML Laboratories Inc. 6918 Ridge Road, Rosedale MD 21237 USA
Tele: (877) 219-5183 Fax: (877) 219-5184